(Terrestre) Comunicaciones de Congresos
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Ítem Acceso Abierto Biofunctionalization of nylon nanofibers to be used in immunobiosensor for biological warfare agents detecting(Formatex Research Center, 2018) Peraile, I.; Lorenzo Lozano, P.; Murillo, N.; Maudes, J.; Rozas Sanz, Gabriel; Pérez Márquez, Ana; González López, L.; Cabria Ramos, J. C.; Gil García, M.The use of biological warfare agents involves a growing threat to society. Thus, the most countries have been forced to increase resources in research for their detection and identification. One of the critical points in the effective fight against these agents is the development of devices that allow their detection and early identification. The best choice is the immunobiosensors easy to use on-site. However, how the antibody is attached to the biosensor surface, in terms of density and orientation, will determine the diagnosis capability of the device. In this study, both a functional nanofiber able to increase the surface / volume relation, and a chemicallysimilar planar membrane were used as support for the immobilization of antibodies. Different antibody immobilization systems were carried out to biofunctionalize both surfaces: passive adsorption, covalent bond by glutaraldehyde and well-oriented immobilization by protein A/G. Our results showed that nanofibers in combination with protein A/G were a very effective immunocapture system for being used in a biosensor.Ítem Acceso Abierto lnactivation and identification of Bacillus anthracis spores(Wageningen Academic Publishers, 2013-12) Gil García, M.; Peraile, I.; Jiménez Pérez, M. V.; Cabria Ramos, J. C.; Lorenzo Lozano, P.Bacillus anthracis endospores must be handled ata biosafety level-3 (BSL-3} eontainment facility, therefore an appropriate inaetivation procedure is essential in order to both detect thcm in a reliable and rapid way and to avoid human infections from antrax-contamined samples if a biosafety level-3 laboratory is not availablc. Unfortunatcly, donnant spores exhibit incredible hardiness against gennicidal agents. The most effective methods for inactivating spores are usually the chemical treatment, heat inactivation (boiling, moist hcat, dry heat) and irTadiation (microwave, UV, gamma, electron beam). The inactivation method used must alter thc cell structure as little as possible in order to ensure a corree! diagnosis by diferent techniques. Several kinds ofphysical and chemical inactivation methods wcrc assessed in order to define the conditions that ensure inactivation of endospores of B. anthracis 34F2 Sterné and pcrmit, at the same time, its identification by PCR and immunoassays techniques.
